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Jafari, A, Pakdel, A, Esmailkhanian, S. (1378). Growth Hormone Gene Polymorphism in Two Iranian Native Fowls (Short Communication). , 3(1), 99-104. doi: 10.22069/psj.1999.2331
A Jafari; A Pakdel; S Esmailkhanian. "Growth Hormone Gene Polymorphism in Two Iranian Native Fowls (Short Communication)". , 3, 1, 1378, 99-104. doi: 10.22069/psj.1999.2331
Jafari, A, Pakdel, A, Esmailkhanian, S. (1378). 'Growth Hormone Gene Polymorphism in Two Iranian Native Fowls (Short Communication)', , 3(1), pp. 99-104. doi: 10.22069/psj.1999.2331
Jafari, A, Pakdel, A, Esmailkhanian, S. Growth Hormone Gene Polymorphism in Two Iranian Native Fowls (Short Communication). , 1378; 3(1): 99-104. doi: 10.22069/psj.1999.2331

Growth Hormone Gene Polymorphism in Two Iranian Native Fowls (Short Communication)

Poultry Science Journal
مقاله 10، دوره 3، شماره 1، فروردین 2015، صفحه 99-104    اصل مقاله (270.06 K)
نوع مقاله: Short Communication
شناسه دیجیتال (DOI): 10.22069/psj.1999.2331
نویسندگان
A Jafari1؛ A Pakdel* 2؛ S Esmailkhanian3
1Department of Animal Sciences, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
2Department of Animal Sciences, Isfahan University of Technology, Isfahan, Iran.
3Isfahan Research Center for Agriculture and Natural Resources, Isfahan, Iran.
چکیده
Biochemical polymorphism study is a method of determination of genetic variation. This variability could be a basis for selection and subsequent genetic improvement in farm animals. The polymorphism in the intron 1 of chicken growth hormone (cGH) gene was investigated in the Iranian native fowls by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. The genomic DNA was extracted from 217 samples (129 samples from the native fowls of Isfahan province and 88 samples from the native fowls of Mazandaran province) by using modified salting out technique. The DNA fragment of the growth hormone gene with 776 bp was amplified by PCR using specific primers. Then the PCR products were digested with MspI restriction enzyme and analyzed on 2.5% agarose gel. The allelic frequency of intron 1 locus for A1, A2 and A3 alleles in  Isfahan native fowls were 0.60, 0.21 and 0.19 and those in Mazandaran native  fowls were 0.28, 0.05 and 0.67, respectively. The results of current study indicated that the intron 1 of cGH is polymorphic in Iranian native fowls and could be exploited as a candidate gene for marker-assisted selection for growth-related traits.
مراجع
Egena SSA & Alao RO. 2014. Haemoglobin polymorphism in selected farm animal: A review. Biotechnology in Animal Husbandry, 30: 377-390.

Feng XP, Kuhnlein U, Aggrey SE, Gavora JS & Zadworny D. 1997. Trait association of genetic markers in the growth hormone and the growth hormone receptor gene in a White Leghorn strain. Poultry Science, 76: 1770-1775.

Fotouhi N, Karatzas CN, Kuhlein U & Zadworny D. 1993. Identification of growth hormone DNA polymorphisms which response to divergent selection for abdominal fat content in chickens. Theoretical and Applied Genetics, 85: 931–936.

Ghelghachi AA, Seyedabadi HR & Lak A. 2013. Association of growth hormone gene polymorphism with growth and fatness traits in Arian broilers. International Journal of Biosciences, 3: 216-220.

Ip SC, Zhang X & Leung FC. 2001. Genomic growth hormone gene polymorphisms in native Chinese chickens. Experimental Biology and Medicine, 226: 458-462.

Kansaku N, Hiyama G, Sasanami T & Zadworny D. 2008. Prolactin and growth hormone in birds: Protein structure, gene structure and genetic variation. Journal of Poultry Science, 45: 1-6.

Krasnopiorova N, Baltrenaite L & Miceikien I. 2012. Growth hormone polymorphism and its influence on milk traits in cattle bred in Lithuania. Veterinarija ir Zootecknika, 58: 42-46.

Kuhnlein U, Ni L, Zadworny D, Weigend S, Gavora JS & Fairfull W. 1997. DNA polymorphisms in the chicken growth hormone gene: response to selection for disease resistance and association with egg production. Animal Genetics, 28: 116-123.

Lechniak D, Machnik G, Szydlowski M & Switonski M. 1999. Growth hormone gene polymorphism and reproductive performance in AI bulls. Theriogenology, 52: 1145–1152.

Miller SA, Dykes DD & Polesky HF. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16: 1215.

Osterhoff DR. 1964. Recent research on biochemical polymorphism in livestock. Journal of South African Veterinary Medicine Association, 35: 363-380.

Shaw EM, Shoffner RN, Foster DN & Guise KS. 1991. Mapping of the growth hormone gene by in situ hybridization to chicken chromosome 1. Journal of Heredity, 82: 505-508.

Sheng-Long W, Musa HH, Wen-Bin B, Ke-Hua W, Guo-Qian Z & Guo-Hong C. 2008. Mutation in exon 4 apo VLDL-II gene is a candidate for meat tenderness in chickens. Journal of Animal and Veterinary Advances, 7: 1624-1627.

Su YJ, Shu JT, Zhang M, Zhang XY, Shan YJ, Li GH, Yin JM, Song WT, Li HF & Zhao GP. 2014. Association of chicken growth hormone polymorphisms with egg production. Genetics and Molecular Research, 13: 4893- 4903.

Tanaka M, Hosokawa Y, Watahiki M & Nakashima K. 1992. Structure of the chicken growth hormone-encoding gene and its promoter region. Gene, 112: 235–239.

Please cite this article as: Jafari A, Pakdel A & Esmailkhanian S. 2015. Growth hormone gene polymorphism in two Iranian native fowls. Poult. Sci. J. 3 (1): 99-104
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