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Effect of Heavy metals Fe, Cu, and Zn on Soil Free-Living N-Fixing Bacteria | ||
| Environmental Resources Research | ||
| دوره 13، شماره 2، دی 2025، صفحه 251-260 اصل مقاله (1.39 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22069/ijerr.2025.23287.1479 | ||
| نویسندگان | ||
| Nasrin Sabour Moghaddam* 1؛ Paniz Abdollahi Saeed2 | ||
| 1Department of Environmental and Natural Resources, Payame Noor University, Tehran, Iran | ||
| 2Plant Protection Department, Faculty of Agriculture, University of Tabriz, Tabriz, Iran | ||
| چکیده | ||
| Nitrogen-fixing bacteria play a crucial role in sustainable agriculture by enhancing soil fertility and reducing the need for chemical fertilizers. However, heavy metal pollution, particularly from iron (Fe), copper (Cu), and zinc (Zn), poses a significant threat to these beneficial microorganisms. This study investigates the effects of three heavy metals - iron (Fe), copper (Cu), and zinc (Zn) - on the populations of two free-living nitrogen-fixing bacterial strains, Azospirillum irakense (AR) and Azotobacter chroococcum (AC), under controlled laboratory conditions. The experimental treatments included sulfate ion (SO4-) solutions of Fe, Cu, and Zn at four concentrations (0.1, 1, 10, and 100 mg/L), alongside a control group. The results revealed that Zn had the most pronounced inhibitory effect, with population reductions of up to 76% for AR and 80% for AC at the highest concentration of 100 mg/L. Cu also exhibited significant inhibitory effects, particularly at higher concentrations, reducing AR and AC populations by 28% and 15%, respectively, at 100mg/L. Lower concentrations of Cu (0.1 and 1mg/L) showed no significant impact on the bacterial populations. In contrast, Fe did not demonstrate any significant inhibitory effects across all tested concentrations. Additionally, we investigated the accumulation of heavy metals within bacterial cells and the production of reactive oxygen species under metal stress. Both experiments revealed significant intracellular metal accumulation and elevated ROS levels at higher metal concentrations, further elucidating the mechanisms of metal toxicity. These findings highlight the potential risks associated with the accumulation of heavy metals, particularly Zn and Cu, in agricultural soils. | ||
| کلیدواژهها | ||
| Azospirillum irakense؛ Azotobacter chroococcum؛ Sustainable agriculture | ||
| مراجع | ||
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