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Sanganagouda, K, Naveen, AN, Shivaraj, M, Dhinakarraj, G. (1392). Humoral Immune Response Induced by PLGA Micro Particle Coupled Newcastle Disease Virus Vaccine in Chickens. , 2(1), 15-24. doi: 10.22069/psj.2014.1496
K Sanganagouda; AN Naveen; M Shivaraj; G Dhinakarraj. "Humoral Immune Response Induced by PLGA Micro Particle Coupled Newcastle Disease Virus Vaccine in Chickens". , 2, 1, 1392, 15-24. doi: 10.22069/psj.2014.1496
Sanganagouda, K, Naveen, AN, Shivaraj, M, Dhinakarraj, G. (1392). 'Humoral Immune Response Induced by PLGA Micro Particle Coupled Newcastle Disease Virus Vaccine in Chickens', , 2(1), pp. 15-24. doi: 10.22069/psj.2014.1496
Sanganagouda, K, Naveen, AN, Shivaraj, M, Dhinakarraj, G. Humoral Immune Response Induced by PLGA Micro Particle Coupled Newcastle Disease Virus Vaccine in Chickens. , 1392; 2(1): 15-24. doi: 10.22069/psj.2014.1496

Humoral Immune Response Induced by PLGA Micro Particle Coupled Newcastle Disease Virus Vaccine in Chickens

Poultry Science Journal
مقاله 3، دوره 2، شماره 1، فروردین 2014، صفحه 15-24    اصل مقاله (403.17 K)
نوع مقاله: Original Paper
شناسه دیجیتال (DOI): 10.22069/psj.2014.1496
نویسندگان
K Sanganagouda* 1؛ AN Naveen2؛ M Shivaraj1؛ G Dhinakarraj3
1Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore. Karnataka State. India.
2Veterinary Dispensary, Dabbegatta Kaimara, Madhugiri Tq, Tumkur Dist. Karnataka. India.
3Translational Research Platform for Veterinary Biologicals, TANUVAS, Chennai. Tamilnadu, India.
چکیده
This experiment was conducted for evaluating the humoral immune responses induced by Poly Lactide-co-Glycolide Acid (PLGA) microspheres coupled inactivated Newcastle Disease Virus (NDV) vaccine in comparison to an ‘in-house’ prepared inactivated and a live commercial vaccine. PLG microparticles containing inactivated NDV were prepared by a double emulsion technique based on solvent evaporation method. The size of the NDV coupled PLG microparticles was determined by Electron Microscopy. NDV coupled PLG microparticles were spherical having smooth surface, hollow core inside with no pores on the surface. The experiment was conducted in four groups of chickens (n=15). The encapsulation efficiency of NDV coupled PLG microparticles was determined by protein estimation and HA activity in elute. The mean (± SE) size of PLG microspheres was found to be 2.409 ± 0.65 µm. The mean percent of encapsulation efficiency of PLG microspheres coupled to NDV was assessed based on the total protein content and HA activity in elute was found to be 8.03 ± 0.50 and 12.5 ± 0.00, respectively. In conclusion, the results of the experiment showed that PLGA coupled NDV vaccine elicited stronger and prolonged humoral immune response in chickens, in comparison to the other tested vaccines, as assessed by haemagglutination inhibition and enzyme linked immuno sorbent asaay titers.
کلیدواژه‌ها
ELISA؛ PLG microsphere؛ Newcastle disease vaccine؛ Haemagglutination inhibition
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Please cite this article as: Sanganagouda K, Naveen AN, Shivaraj M & Dhinakarraj G . 2014. Humoral immune response induced by PLGA micro particle coupled Newcastle disease virus vaccine in chickens. Poult. Sci. J. 2 (1): 15-24
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