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بهینه سازی استخراج ترکیبات ضداکسیدانی جلبک قهوه ای Sargassum angustifoliumبا رفلاکس حرارتی در روش تاگوچی | ||
| مجله بهره برداری و پرورش آبزیان | ||
| مقاله 1، دوره 5، شماره 3، مهر 1395، صفحه 1-13 اصل مقاله (309.83 K) | ||
| شناسه دیجیتال (DOI): 10.22069/japu.2017.12858.1342 | ||
| نویسندگان | ||
| آریا باباخانی لشکان* 1؛ زهرا نحوی2 | ||
| 1دانشگاه گیلان | ||
| 2تربیت مدرس | ||
| چکیده | ||
| در این مطالعه از روش رفلاکس حرارتی برای استخراج عصاره از جلبک قهوه ای سارگاسوم استفاده شد. استخراج با 3 حلال (اتانول، آب/اتانول و آب) و در 3 زمان (90، 180 و 270 دقیقه) انجام شد. آزمایش‌ها با استفاده از طرح تاگوچی در سه سطح انجام شد. میزان فنول کل و خنثی کنندگی رادیکال آزاد سنجیده شد. با توجه به نتایج، بهترین شرایط برای استخراج ترکیبات ضداکسیدانی از سارگاسوم استخراج آبی و زمان 270 دقیقه بود. میزان فنول کل و IC50 در شرایط بهینه 392 میلی گرم اسید گالیک بر گرم ماده خشک (GAE) و 008/0 میلی گرم عصاره بود. نتایج نشان داد که این جلبک برای استخراج ترکیبات ضداکسیدانی مناسب می باشد. | ||
| کلیدواژه‌ها | ||
| سارگاسوم؛ ضداکسیدان‌های طبیعی؛ خلیج فارس | ||
| مراجع | ||
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