آمار
| تعداد نشریات | 13 |
| تعداد شمارهها | 656 |
| تعداد مقالات | 6,849 |
| تعداد مشاهده مقاله | 9,880,321 |
| تعداد دریافت فایل اصل مقاله | 9,245,478 |
Cryopreservation and Biological Characteristics of Bangkok Chicken (Thai Game Fowl) Embryonic Fibroblast Cell Culture | ||
| Poultry Science Journal | ||
| دوره 12، شماره 1، خرداد 2024، صفحه 65-73 اصل مقاله (1.32 M) | ||
| نوع مقاله: Original Paper | ||
| شناسه دیجیتال (DOI): 10.22069/psj.2023.21639.1966 | ||
| نویسندگان | ||
| Elisa Herawati* ؛ Gafia Ratna Gayatri | ||
| Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Jl. Ir. Sutami 36 A, Surakarta, Central Java, Indonesia | ||
| چکیده | ||
| Bangkok chicken, renowned for its exceptional characteristics, presents a valuable option for crossbreeding to enhance the local chicken varieties in Indonesia. Cryopreservation is widely considered a promising approach for protecting the genetic diversity of the Bangkok chicken. This research aims to investigate the biological characteristics of embryonic fibroblast cells from Bangkok chickens, with the goal of preserving the chicken strain's genetic diversity through cryopreservation methods. The fibroblast cells were derived from 8–10-day-old Bangkok chicken embryos and cultivated in DMEM media. Growth curves and Population Doubling Time were observed to assess the rate of fibroblast cell growth. Immunocytochemistry staining was employed to examine the protein expression associated with fibroblast cell adhesion. The results showed that fibroblast cells predominantly exhibited a fusiform morphology rather than an elongated cytoplasmic morphology. The cells proliferated continuously with an average population doubling time of 204 h and the maximum cell count was 7.83×104±1.7 cells/ml. The cells exhibited normal detection of protein expression, including integrin, cadherin, actin, and microtubules. It was also noted that after cryopreservation in liquid nitrogen, fibroblast cells took 4-6 days to grow and reach 80% confluency. The data collectively verifies the successful establishment of cell culture and cryopreservation for Bangkok chicken embryonic fibroblast cells. | ||
| کلیدواژهها | ||
| Bangkok chicken؛ fibroblast؛ cryopreservation؛ cell culture | ||
| مراجع | ||
|
Bai C, Wang D, Li C, Jin D, Li C, Guan W & Ma Y. 2011. Establishment and biological characteristics of a Jingning chicken embryonic fibroblast bank. European Journal of Histochemistry, 55(1):19-23. DOI: 10.4081/ejh.2011.e4
Bianconi D, Unseld M & Prager GW. 2016. Integrins in the spotlight of cancer. International Journal of Molecular Sciences, 17 (2037): 1-27. DOI: 10.3390/ijms17122037
Blackburn HD. 2018. Biobanking genetic material for agricultural animal species. Annual Review of Animal Biosciences, 6: 69-82. DOI: 10.1146/annurev-animal-030117-014603
Boucher L. 2005. What is doubling time and how is it calculated?. populationeducation.org/what-doubling-time-and-how-it-calculated/ Accessed on May 4. 2023.
Bucala R. 2007. Fibrocytes: new insights into tissue repair and systemic fibroses. 1st Ed. World Scientific Publishing Company. Singapore. 268 Pages.
Budiharjo A, Prabawati AWF & Herawati E. 2021. Seroprevalence of avian influenza virus subtype H5N1 in chickens of the two main poultry market in Surakarta area, Central Java. IOP Conference Series: Earth and Environmental Science, 902: 012035. DOI:10.1088/1755-1315/902/1/012035
Eyden B. 2004. Fibroblast phenotype plasticity: relevance for understanding heterogeneity in “fibroblastic” tumors. Ultrastructural Pathology, 28: 307-319. DOI: 10.1080/019131290882204
Fernandes IR, Russo FB, Pignatari GC, Evangelinellis MM, Tavolari S, Muotri AR & Beltrão-Braga PC. 2016. Fibroblast sources: where can we get them?. Cytotechnology, 68(2): 223–228. DOI: 10.1007/s10616-014-9771-7
GenRes Bridge. 2021. Animal genetic resources strategy for Europe. https://www.animalgeneticresources.net/wp-content/uploads/2021/11/AnGR_strategy_FinalDraft2111.pdf. Accessed in October, 2022.
Freshney RI. 2011. Culture of animal cells : A manual of basic technique and specialized applications 6th Ed. John Wiley & Sons, Inc. New Jersey. 732 Pages.
Guan W, He X, Li L, Liang H, Zhao Q, Pu Y, & Ma YH. 2010. Establishment and biological characterization of fibroblast cell line from the Langshan chicken. Cell Proliferation, 43(2), 157–163. DOI: 10.1111/j.1365-2184.2010.00666.x
Guan W, Wang D, Bai C, Zhang M, Li C & Ma Y. 2012. Biological characteristic of an embrionic fibroblast line from Xiaoshan chicken for genetic conservation. Journal of Cell and Animal Biology, 6(4): 46-53. DOI: 10.5897/JCAB11.089
Herawati E, Wulandari H, Listyawati S & Etikawati N. 2021. In vitro fibroblast cells culture from Pelung chicken embryo and its potential application. IOP Conference Series: Earth and Environmental Science, 637(1): 012007. DOI: 10.1088/1755-1315/637/1/012007
IUCN. 2020. The IUCN red list of threatened species. Accessed on December 2. 2020.
Jang TH, Park SC, Yang JH, Kim JY, Seok JH, Park US, Choi CW, Lee SR & Han J. 2017. Cryopreservation and its clinical applications. Integrative Medicine Research, 6: 12-18. DOI: 10.1016/j.imr.2016.12.001
Li IF, Guan WJ, Li H, Bai XJ & Ma YH. 2009. Establishment and characterization of the fibroblast line from Silkie Bantam. Asian Australian Journal Animal Science, 22(4):492-499. DOI: 10.5713/ajas.2009.80480
Liu S & Li F. 2020. Cryopreservation of single-sperm: where are we today?. Reproductive Biology and Endocrinology, 18(1): 41. DOI: 10.1186/s12958-020-00607-x
Luo S, Xie Z, Xie Z, Xie L, Huang L, Huang J, Deng X, Seng T, Wang S, Zang Y & Liu J. 2017. Surveillance of live poultry markets for low pathogenic avian influenza viruses in Guangxi Province, Southern China, from 2012–2015. Scientific Reports, 7: 17577. DOI: 10.1038/s41598-017-17740-0
Mahesh YU, Rao BS, Katari VC, Komjeti S, Christo D, Lakshmikantan U, Pawar RM & Shivaji S. 2012. Cell cycle synchronization of bison (Bos Gaurus) fibroblasts derived from ear piece collected post-mortem. Reproduction in Domestic Animals, 47: 799-805. DOI: 10.1111/j.1439-0531.2011.01970.x
Maitre JL & Heisenberg CP. 2013. Three functions of cadherins in cell adhesion. Current Biology, 23 (14): 626-633. DOI: 10.1016/j.cub.2013.06.019
Maliwan P, Khempaka S & Molee W. 2017. Evaluation of various feeding programmes on growth performance, carcass and meat qualities of Thai indigenous crossbred chickens. South African Journal of Animal Science, 47(1): 16-25. DOI: 10.4314/sajas.v47i1.4
Manso AM, Kang SM & Ross RS. 2010. Integrins, focal adhesion, and cardiac fibroblasts. Journal of Investigative Medicine, 57 (8): 856-860. DOI: 10.231/JIM.0b013e3181c5e61f
Master JR & Stacey GN. 2007. Changing medium and passaging cell lines. Nature Protocols, 2(9): 2276-2284. DOI: 10.1038/nprot.2007.319
Mehrabani D, Tajedini M, Tamadon A, Dianatpour M, Parvin F, Zare S & Rahmanifar F. 2016. Establishment, characterization and cryopreservation of fars native goat fetal fibroblast cell lines. Asian Pacific Journal of Reproduction, 5(3): 247-251. DOI: 10.1016/j.apjr.2016.04.013
Mokodongan AR, Nangoy F, Leke JR & Poli Z. 2017. Penampilan pertumbuhan ayam Bangkok starter yang diberi pakan dengan level protein berbeda. Jurnal Zootek, 37(2): 426-435. [INDONESIAN]. DOI: 10.35792/zot.37.2.2017.16268
Na RS, Bai CY, Jin DP, Su XH, Feng, BG, Guan, WJ & Ma YH. 2010. Establishment and biological characteristics of Qingyuan partridge chicken fibroblast line. Poultry Science, 89(6), 1207–1216. DOI: 10.3382/ps.2009-00558
Pasitka L, Cohen M, Ehrlich A, Gildor B, Reuveni E, Ayyash M, Wissotsky G, Herscovici A, Kaminker R, Niv A, Bitcover R, Dadia O, Rudik A, Voloschin A, Shimoni M, Cinnamon Y & Nahmias Y. 2022. Spontaneous immortalization of chicken fibroblasts generates stable, high-yield cell lines for serum-free production of cultured meat. Nature Food, 4: 35–50. DOI: 10.1038/s43016-022-00658-w
Rakha BA, Ansari, MS, Akhter S, Hussain I & Blesbois E. 2016. Cryopreservation of Indian red jungle fowl (Gallus gallus murghi) semen. Animal Reproduction Science, 174: 45-55. DOI: 10.1016/j.cryobiol.2017.07.006
Ramage L. 2012. Integrins and extracellular matrix in mechanotransduction. Cell Health and Cytoskeleton, 4: 1-9. DOI: 10.1101/cshperspect.a005066
Repiská V, Varga I, Lehocký I, Böhmer D, Blaško M, Polák Š, Adamkov M & Danišoviče Ľ. 2010. Biological and morphological characterization of human neonatal fibroblast cell culture B-HNF-1. Biologia, 65(5): 919-924. DOI: 10.2478/s11756-010-0095-6
Santiago-Moreno J & Blesbois E. 2022. Animal board invited review: germplasm technologies for use with poultry. Animal, 16(3): 100475. DOI: 10.1016/j.animal.2022.100475
Su X, Jin D, Li C, Yue H, Pu Y, Ma Y & Guan W. 2011. Establishment of big bone chicken fibroblast cell bank and study of its biological characteristics. Belgian Journal of Zoology, 141 (1): 14-23.
Sulandari S, Zein MSA & Sartika T. 2008. Molecular characterization of Indonesian indigenous chickens based on mitochondrial DNA displacement (D)-loop sequences. Hayati Journal of Biosciences, 15(4): 145-154. DOI: 10.4308/hjb.15.4.145
Taye S, Goshu G & Abegaz S. 2022. Effect of crossbreeding on growth performance of improved Horro Crosses with Koekoek and Kuroiler chicken breeds. Poultry Science Journal, 10(1): 35-44. DOI: 10.22069/psj.2022.19503.1729
Tracy LE, Minasian RA & Caterson EJ. 2016. Extracellular matrix and dermal fibroblast function in the healing wound. Advance Wound Care, 5(3): 119-136. DOI: 10.1089/wound.2014.0561
Winaya A, Fahmiady DI, Suyatno S, Malik AM & Jaganathan R. 2023. Morphometric diversity and genetic relationship of “Bangkok” chicken (Thai Game Fowl) in East Java, Indonesia. Jordan Journal of Biological Sciences, 16 (2): 189 -197. DOI: 10.54319/jjbs/160203 | ||
|
آمار تعداد مشاهده مقاله: 484 تعداد دریافت فایل اصل مقاله: 466 |
||
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب